Case study involving wound closure of two large complex wounds using autologous engineered skin graft grown from 1 cm2 area of patient's skin

Case 1The patient is a 80 year old male with a significant past medical history of rheumatoid arthritis, pulmonary coccidiomycosis, chronic kidney disease – stage III, coronary artery disease of native vessel and chronic anticoagulation with direct oral anticoagulant secondary to history of deep vein thrombosis/pulmonary embolism who presented to Banner Del E Webb Medical Center with a left thigh hematoma The patient was found to have an infected left thigh hematoma. The patient underwent on 7/22/2021 an excisional debridement of the right medial thigh wound with drainage of hematoma. The patient was treated initially with negative pressure wound therapy with instillation and dwell followed by conventional negative pressure Once the patient had healthy granulation tissue, the patient underwent the MyOwnSkin procedure:1 cm squared area of epidermis + dermis excised from the left shoulder area plus 12 vials of blood. This was sent to BioLab Sciences in Scottsdale, AZ where in 5 days the requested skin graft was created using the patient's own skin. The patient underwent successful application of the skin graft after an amniotic liquid allograft was injected in various areas of the wound bedCase 2The patient is a 65 year old female with a significant past medical history of chronic venous insufficiency and secondary lower extremity lymphedema who has a chronic venous ulcer of the right calf for over 4 years. After obtaining adequate venous compression using 2 layer venous compression wraps and lymphedema pumps routinely, the patient had marked improvement of the lower extremity edema. Once the patient had healthy granulation tissue, a 1 cm2 area of skin was excised from patient's left shoulder area and 12 vials of blood which were sent to BioLab Sciences in Scottsdale, AZ. Within 5 days, an allograft of the requested size was shipped back for application. An amniotic liquid allograft was injected in various areas of the wound bed followed by application of the patient's autologous skin graft. The patient has nearly healed with a small area remaining of granulation tissue. The above two procedures again were done in the office setting No significant pain